2016

Signaling through Lrg1, Rho1 and Pkc1 Governs Candida albicans Morphogenesis in Response to Diverse Cues. 2016. Xie, J.L., Grahl, N., Sless, T., Leach, M.D., Kim, S.H., Hogan, D.A., Robbins, N., and Cowen, L. E. PLoS Genetics 12:e1006405.

Metal Chelation as a Powerful Strategy to Probe Cellular Circuitry Governing Fungal Drug Resistance and Morphogenesis. Polvi, E.J., Averette, A.F., Lee, S.C., Kim, T., Bahn, Y.S., Veri, A.O., Robbins, N., Heitman, J., and Cowen, L.E. PLoS Genetics 12:e1006350.

Dual Action Antifungal Small Molecule Modulates Multidrug Efflux and TOR Signaling. 2016. Shekhar-Guturja, T., Gunaherath, G.M., Wijeratne, E.M., Lambert, J.P., Averette, A.F., Lee, S.C., Kim, T., Bahn, Y.S., Tripodi, F., Ammar, R., Döhl, K., Niewola-Staszkowska, K., Schmitt, L., Loewith, R.J., Roth, F.P., Sanglard, D., Andes, D., Nislow, C., Coccetti, P., Gingras, A.C., Heitman, J., Gunatilaka, A.A., and Cowen, L.E. Nature Chemical Biology 12:867-75.

Mapping the Hsp90 Genetic Network Reveals Ergosterol Biosynthesis and Phosphatidylinositol-4-Kinase Signaling as Core Circuitry Governing Cellular Stress.
2016. O'Meara, T.R., Veri, A.O., Polvi, E.J., Li, X., Valaei, S.F., Diezmann, S., and Cowen, L.E. PLoS Genetics 12:e1006142.

Hsf1 and Hsp90 Orchestrate Temperature-Dependent Global Transcriptional Remodelling and Chromatin Architecture in Candida albicans. 2016. Leach, M. D., Farrer, R. A., Tan, K., Miao, Z., Walker, L. A., Cuomo, C.A., Wheeler, R. T., Brown, A. J. P., Wong, K. H., Cowen, L. E. Nature Communications 7:11704.

Antifungal Drugs: The Current Armamentarium and Development of New Agents. 2016. Robbins, N., Wright, G.D., and Cowen, L.E. Microbiology Spectrum 4(5). doi: 10.1128/microbiolspec.FUNK-0002-2016.

Beauvericin Potentiates Azole Activity via Inhibition of Multidrug Efflux, Blocks C. albicans Morphogenesis, and is Effluxed via Yor1 and Circuitry Controlled by Zcf29. 2016. Shekhar-Guturja, T., Tebung, W.A., Mount, H., Liu, N., Köhler, J.R., Whiteway, M., and Cowen, L.E. Antimicrobial Agents & Chemotherapy Oct 10. pii: AAC.01959-16.

Candida albicans Is Resistant to Polyglutamine Aggregation and Toxicity. 2016. Leach, M.D., Kim, T., DiGregorio, S., Collins, C., Zhang, Z., Duennwald, M.L., and Cowen, L.E. G3 Nov 2. pii: g3.116.035675. doi: 10.1534/g3.116.035675.

2015

Global Analysis of the Fungal Microbiome in Cystic Fibrosis Patients Reveals Loss of Function of the Transcriptional Repressor Nrg1 as a Mechanism of Pathogen Adaptation. Kim, S. H., Clark, S. T., Surendra, A., Copeland, J., Wang, P., Ammar, R., Collins, C., Tullis, D. E., Nislow, C., Hwang, D. M., Guttman, D. S., and Cowen, L. E. 2015. PLoS Pathogens 11(11):e1005308.

Functional Divergence of Hsp90 Genetic Interactions in Biofilm and Planktonic Cellular States. Diezmann, S.*, Leach, M. D.*, and Cowen, L. E. 2015. PLoS ONE 10(9):e0137947.

Global Analysis of Fungal Morphology Exposes Mechanisms of Host Cell Escape. O'Meara, T. R.*, Veri, A.*, Ketela, T., Jiang, B., Roemer, T., and Cowen, L. E. 2015. Nature Communications 6:6741.

Fitness Trade-Offs Associated with the Evolution of Resistance to Antifungal Drug Combinations. Hill, J. A., O’Meara, T. R., and Cowen, L. E. 2015. Cell Reports 10:809-19.
See accompanying Highlight in
Nature Reviews Microbiology 13: doi:10.1038 (2015).

Opportunistic Yeast Pathogens: Reservoirs, Virulence Mechanisms, and Therapeutic Strategies. Polvi, E. J.*, Li, X.*, O'Meara, T. R., Leach, M. D., Cowen, L. E. 2015. Cellular and Molecular Life Sciences Feb 21. [Epub ahead of print] PMID: 25700837.

Using Combination Therapy to Thwart Drug Resistance. Hill, J. A. and Cowen, L. E. 2015. Future Microbiology 10:1719-26.

Drug Combinations as a Strategy to Potentiate Existing Antifungal Compounds. In Antifungals: From Genomics to Resistance and the Development of Novel Agents. Sanglard, D. and Cowen, L. E. 2015. Edited by: Alix T. Coste and Patrick Vandeputte. Horizon Caister Academic Press. Linton, UK.

2014

Membrane Fluidity and Temperature Sensing are Coupled via Circuitry Comprised of Ole1, Rsp5, and Hsf1 in Candida albicans. Leach, M.D. and Cowen LE. 2014. Eukaryotic Cell 13(8):1077-84.

Hsp90-dependent Regulatory Circuitry Controlling Temperature-dependent Fungal Development and Virulence. O'Meara, T. R., and Cowen, L. E. 2014. Cellular Microbiology 16(4):473-81.

Mechanisms of Antifungal Drug Resistance. Cowen, L. E., Sanglard, D., Howard, S. J., Rogers, P. D., and Perlin, D. S. 2014. Cold Spring Harbor Perspectives in Medicine doi: 10.1101/cshperspect.a019752. [Epub ahead of print]. PMID: 25384768.

Elucidating Drug Resistance in Human Fungal Pathogens. Xie, J. L.*, Polvi, E. J.*, Shekhar-Guturja, T.*, Cowen, L. E. 2014. Future Microbiology 9:523-42.

Progress and Prospects for Targeting Hsp90 to Treat Fungal Infections. Veri, A. and Cowen, L. E. 2014. Parasitology 141(9):1127-37.

To Sense of Die: Mechanisms of Temperature Sensing in Fungal Pathogens. Leach, M. D. and Cowen, L. E. 2014. Current Fungal Infection Reports 8:185-191.

Microevolution of Antifungal Drug Resistance. Cowen, L. E. 2014. In Handbook of Antimicrobial Resistance. Edited by Matthias Gotte, Albert Berghuis, Greg Matlashewski, Mark Wainberg and Donald Sheppard. Springer. New York.

2013

Genetic and Genomic Architecture of the Evolution of Resistance to Antifungal Drug Combinations. Hill, J. A., Ammar, R., Torti, D., Nislow, C., and Cowen L. E. 2013. PLoS Genetics 9(4): e1003390.

The Fungal Achilles' Heel: Targeting Hsp90 to Cripple Fungal Pathogens. Cowen, L. E. 2013. Current Opinion in Microbiology doi:pii: S1369-5274(13)00037-4. Epub ahead of print.

Surviving the Heat of the Moment: a Fungal Pathogens Perspective. Leach, M. D. and Cowen, L. E. 2013. PLoS Pathogens 9(3):e1003163.

The Candida albicans Hsp90 Chaperone Network is Environmentally Flexible and Evolutionarily Divergent. Diezmann, S. and Cowen, L. E. 2013. In The Molecular Chaperones Interaction Networks in Protein Folding and Degradation. Edited by W. Houry. Springer. Baton Rouge, LA, USA. P. 185-204.

2012

Hsp90 Coordinates Short Term Transcriptional Regulation of Hsf1 and Longer Term Cell Wall Remodelling by MAP Kinase Signalling During Thermal Adaptation in a Pathogenic Yeast. Leach, M. D., Budge, S., Walker, L., Munro, C., Cowen, L. E., and Brown, A. J. P. 2012. PLoS Pathogens 8(12): e1003069.

Global Gene Deletion Analysis Exploring Yeast Filamentous Growth. Ryan, O.,# Shapiro, R. S.,# Kurat, C. F., Mayhew, D., Baryshnikova, A., Chin, B., Lin, Z.-Y., Cox,, M., Vizeacoumar, F., Cheung, D., Bahr, S., Tsui, K., Tebbji, F., Sellam, A., Istel, F., Schwarzmuller, Reynolds, T. B., Kuchler, K., Gifford, D. K., Whiteway, M., Giaever, G., Nislow, C., Costanzo, M., Gingras, A.-C., Mitra, R. D., Andrews, B., Fink, G. R.,* Cowen, L. E.,* and Boone, C.* 2012. Science 337: 1353-6. #Co-first author. *Co-corresponding author.

Lysine Deacetylases Hda1 and Rpd3 Regulate Hsp90 Function Thereby Governing Fungal Drug Resistance. Robbins, N., Leach, M. D., and Cowen L. E. 2012. Cell Reports 2:878-88.

Regulatory Circuitry Governing Morphogenesis in Saccharomyces cerevisiae and Candida albicans. Shapiro, R. S., Ryan, O., Boone, C., and Cowen, L. E. 2012. Cell Cycle 11(23):4294-5.

Sgt1 Governs Candida albicans Morphogenesis via the Adenylyl Cyclase Cyr1 and Drug Resistance via Calcineurin Signaling. Shapiro, R. S., Zaas, A. K., Betancourt-Quiroz, M., Perfect, J. R., and Cowen L. E. PLoS ONE 7(9):e44734.

Thermal Control of Microbial Development and Virulence: Molecular Mechanisms of Microbial Temperature Sensing. Shapiro, R. S. and Cowen, L. E. 2012. mBio 3(5). doi:pii: e00238-12.

Fungal Hsp90 – A Biological Transistor that Tunes Cellular Outputs to Thermal Inputs. Leach, M. D., Klipp, E., Cowen, L. E., and Brown, A. J. P. 2012. Nature Reviews Microbiology 10: 693-704.

A Novel Calcineurin-Independent Activity of Cyclosporin A in Saccharomyces cerevisiae. Singh-Babak, S. D., Shekhar, T., Smith, A. M., Giaever, G., Nislow, C., and Cowen, L. E. 2012. Molecular Biosystems 8(10):2575-84.

Global Analysis of the Evolution and Mechanism of Echinocandin Resistance in Candida glabrata. Singh-Babak, S. D., Babak, T., Diezmann, S., Hill, J. A., Xie, J. L., Chen, Y.-L., Poutanen, S. M., Rennie, R. P., Heitman, J., and Cowen,, L. E. 2012. PLoS Pathogens 8(5): e1002718.

Pho85, Pcl1 and Hms1 Signaling Governs Candida albicans Morphogenesis Induced by High Temperature or Hsp90 Compromise. Shapiro, R. S., Sellam, A., Tebbji, F., *Whiteway, M., *Nantel, A., and Cowen, L. E. 2012. Current Biology 22(6): 461-70.
See accompanying Dispatch in
Current Biology 22: R225-7 (2012).

Mapping the Hsp90 Genetic Interaction Network in Candida albicans Reveals Environmental Contingency and Rewired Circuitry. Diezmann, S., Michaut, M., Shapiro, R. S., Bader, G. D., and Cowen, L. E. 2012. PLoS Genetics 8(3): e1002562.

Cdc28 Provides a Molecular Link Between Hsp90, Cell Cycle Regulation, and Morphogenesis in the Pathogenic Fungus Candida albicans. Senn, H., Shapiro, R. S., and Cowen, L. E. 2012. Molecular Biology of the Cell 23(2): 268-83.

Uncovering Cellular Circuitry Controlling Temperature-dependent Fungal Morphogenesis. Shapiro, R. S. and Cowen, L. E. 2012. Virulence 3(4):400-4.

The Evolution of Drug Resistance in Fungi. In, Evolution of Virulence in Eukaryotic Microbes. Hill, J. A., Hoot, S. J., White, T. C., and Cowen, L. E. 2012. Editors L. David Sibley, Barbara J. Howlett, and Joseph Heitman. Wiley Blackwell. Somerset, NJ, USA. p. 143 – 167.

2011

A Systems Biology Approach Reveals the Role of a Novel Methyltransferase in Response to Chemical Stress and Lipid Homeostasis. Lissina, E., Young, B., Urbanus, M. L., Guan, X. L., Lowenson, J., Hoon, S., Baryshnikova, A., Riezman, I., Michaut, M., Bader, G., Riezman, H., Cowen, L. E., Wenk, M. R., Clarke, S. G., Giaever, G., and Nislow, C. 2011. PLoS Genetics 7(10): e1002332.

Hsp90 Governs Dispersion and Drug Resistance of Fungal Biofilms. Robbins, N., Uppuluri, P., Nett, J., Rajendran, R., Ramage, G., Lopez-Ribot, J. L., Andes, D., and Cowen, L. E. 2011. PLoS Pathogens 7(9): e1002257.

Regulatory Circuitry Governing Fungal Development, Drug Resistance, and Disease. Shapiro, R. S., Robbins, N., and Cowen, L. E. 2011. Microbiology and Molecular Biology Reviews 75: 213-267.

2010

PKC Signaling Regulates Drug Resistance of the Fungal Pathogen Candida albicans via Circuitry Comprised of Mkc1, Calcineurin, and Hsp90. LaFayette, S. L., Collins, C., Zaas, A. K., Schell, W. A., Betancourt-Quiroz, M., Gunatilaka, A. A. L., Perfect, J. R., and Cowen, L. E. 2010. PLoS Pathogens 6(8): e1001069.

Metabolic Control of Antifungal Drug Resistance. Robbins, N., Collins, C., Morhayim, J., and Cowen, L. E. 2010. Fungal Genetics and Biology 47: 81-93.

Coupling Temperature Sensing and Development: Hsp90 Regulates Morphogenetic Signaling in Candida albicans. Shapiro, R. S. and Cowen, L. E. 2010. Virulence 1: 45-8.

2009

Hsp90 Orchestrates Temperature-Dependent Candida albicans Morphogenesis Via Ras1-PKA Signaling. Shapiro, R. S., Uppuluri, P., Zaas, A. K., Collins, C., Senn, H., Perfect, J. R., Heitman, J., and Cowen, L. E. 2009. Current Biology 19: 621-9.
See accompanying Dispatch in Current Biology 19: R333-4 (2009).
See Highlight in
Nature Reviews Microbiology 7: 405 (2009).

Hsp90 Governs Echinocandin Resistance in the Pathogenic Yeast Candida albicans via Calcineurin. Singh, S. D., Robbins, N., Zaas, A. K., Schell, W. A., Perfect, J. R., and Cowen, L. E. 2009. PLoS Pathogens 5(7): e1000532.

Hsp90 Orchestrates Stress Response Signaling Governing Fungal Drug Resistance. Cowen, L. E. 2009. PLoS Pathogens 5(7): e1000471.

Rapid Acquisition of Aneuploidy Provides Increased Fitness During the Evolution of Antifungal Drug Resistance. Selmecki, A., Dulmage, K., Cowen, L. E., Anderson, J. B., and Berman J. 2009. PLoS Genetics. 5(10):e1000705.

Harnessing Hsp90 Function as a Powerful, Broadly Effective Therapeutic Strategy for Diverse Fungal Infectious Diseases. Cowen, L. E., Singh, S. D., Köhler, J. R., Collins, C., Zaas, A. K., Schell, W. A., Aziz, H., Mylonakis, E., Perfect, J. R., Whitesell, L., and Lindquist, S. 2009. Proceedings of the National Academy of Sciences USA 106: 2818-23.
See accompanying Commentary in
PNAS 106:2971-2 (2009).

2008

The C. albicans Pescadillo Homolog is Required for Hypha-to-Yeast Morphogenesis and Yeast Proliferation. Shen, J., Cowen, L. E., Griffin, A., Chan, L., and Köhler, J. R. 2008. Proceedings of the National Academy of Sciences USA 105: 20918-23.

The Evolution of Fungal Drug Resistance: Modulating Trajectories of Genotype to Phenotype. Cowen, L. E. 2008. Nature Reviews Microbiology 6: 187-98.

Stress, Drugs, and Evolution: The Role of Cellular Signaling in Fungal Drug Resistance. Cowen, L. E. and Steinbach, W. J. 2008. Eukaryotic Cell 7: 747-64.

2006

Genetic Architecture of Hsp90-Dependent Drug Resistance. Cowen, L. E., Carpenter, A. E., Matangkasombut, O., Fink, G. R., and Lindquist, S. 2006. Eukaryotic Cell 5: 2184-8.

2005

Hsp90 Potentiates the Rapid Evolution of New Traits: Drug Resistance in Diverse Fungi. Cowen, L. E. and Lindquist, S. 2005. Science 309: 2185-89.
See accompanying Perspective in
Science 309: 2175-6 (2005).
See Highlight in
Nature Reviews Genetics 6: 801 (2005).
See Highlight in
Nature Reviews Microbiology 3: 908-909 (2005).
See Highlight in
Nature Reviews Drug Discovery 4: 884 (2005).

2004

Mode of Selection and Experimental Evolution of Antifungal Drug Resistance. Anderson, J. B., Sirjusingh, C., Parsons, A., Boone, C., Wickens, C., Cowen, L. E., and Kohn, L. M. 2003. Genetics 163: 1287-98.

2003

Population Genomics of Drug Resistance in Candida albicans. Cowen, L. E., Nantel, A., Whiteway, M. S., Thomas, D. Y., Tessier, D. C., Kohn, L. M., and Anderson, J. B. 2002. Proceedings of the National Academy of Sciences USA 99: 9284-89.

2002

Evolution of Drug Resistance in Candida albicans. Cowen, L. E., Anderson, J. B., and Kohn, L. M. 2002. Annual Review of Microbiology 56: 139-65.

2001

Predicting the Emergence of Resistance to Antifungal Drugs. Cowen, L. E. 2001. FEMS Microbiology Letters 204: 1-7.

Divergence in Fitness and Evolution of Drug Resistance in Experimental Populations of Candida albicans. Cowen, L. E., Kohn, L. M., and Anderson, J. B. 2001. Journal of Bacteriology 183: 2971-78.

Multilocus Genotyping Indicates that the Ability to Invade the Bloodstream is Widespread among Candida albicans Isolates. Luu, L. N., Cowen, L. E., Sirjusingh, C., Kohn, L. M., and Anderson, J. B. 2001. Journal of Clinical Microbiology 39: 1657-60.

Infrequent Genetic Exchange and Recombination in the Mitochondrial Genome of Candida albicans. Anderson, J. B., Wickens, C., Khan M., Cowen, L. E., Federspiel, N., Jones, T., and Kohn, L. M. 2001. Journal of Bacteriology 183: 865-72.

2000

Evolution of Drug Resistance in Experimental Populations of Candida albicans. Cowen, L. E., Sanglard, D., Calabrese, D., Sirjusingh, C., Anderson, J. B., and Kohn, L. M. 2000. Journal of Bacteriology 182: 1515-22.

1999

Multilocus Genotypes and DNA Fingerprints do not Predict Variation in Azole Resistance Among Clinical Isolates of Candida albicans. Cowen, L. E., Sirjusingh, C., Summerbell, R. C., Walmsley, S., Richardson, S., Kohn, L. M., and Anderson, J. B. 1999. Antimicrobial Agents and Chemotherapy 43: 2930-38.